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Chuiko Institute of Surface Chemistry

National Academy of Sciences of Ukraine
(official site)

Center for collective use of scientific instruments / equipment

 

Center for collective use of scientific instruments / equipment "Mass spectrometric complex with laser desorption/ionization MALDI-TOF Autoflex II LRF20" of NAS of Ukraine

 

According to the Order of the Presidium of NAS of Ukraine dated 18.02.05 №104, the Center for Collective Use of Scientific Instruments / Equipment "Mass spectrometric complex with laser desorption / ionization MALDI-TOF Autoflex II LRF20" of the National Academy of Sciences of Ukraine was established at Chuiko Institute of Surface Chemistry of NAS of Ukraine.

 

 

  

For users of the center

 

Center equipment:

 

 

Name of instrument / equipment

 

Main characteristics

Manufacturer, country, and year of manufacture

IMG 20210302 123914

Mass spectrometer Autoflex II LRF20

Time-of-flight mass spectrometer with matrix-assisted laser desorption / ionization (MALDI-TOF / TOF)

Bruker Daltonik GmbH, Germany, 2004

IMG 20210302 123954

HPLC Agilent 1100*

High performance liquid chromatograph for preparative chromatography

Agilent Technologies, Germany, 2004

 * the equipment is in the process of modernization / completion

 

Mass spectrometer Autoflex II LRF20

 

 

Mass spectrometric complex with laser desorption / ionization MALDI-TOF Autoflex II LRF20 is designed for analysis of ions in a wide range of m/z; it provides detailed and accurate information on the molecular weight of the analyte; it allows analyzing a wide range of molecules - from smaller molecules, such as lipids and peptides, to polymers and intact proteins exceeding 100 kDa; it is indispensable in the analysis of non-volatile macromolecular compounds (proteins, carbohydrates, oligonucleotides) and small molecules (secondary plant metabolites, phytohormones, etc.).

 

The main technical characteristics of the Instrument

Mass range:

~ 250 kDa (Linear Mode)

~ 6,000 Da (Reflectron Mode)

Resolution:

>  4,000 @ FWHM* (Linear Mode)

>  10,000 @ FWHM* (Reflectron Mode)

Ionization (±eV):

Matrix Assisted LASER Desorption/Ionization (MALDI) – 337 nm Nitrogen laser

Inlet Systems:

-384 Microtitre Plate/Target

-LASER: 337nm Nitrogen

Features:

-Delayed Extraction (DE))

-Post Source Decay (PSD)

Data System:

-Windows 2000 SP4

-Bruker Compass 1.0, FlexControl 2.2

-FlexAnalysis 2.2 and license

-GUI Integrated vacuum measurement and control unit

Predominant Uses:

-Characterization and identification of proteins by peptide mass mapping

-PSD and peptide sequencing

-Analysis of low molecular weight organic substances

*FWHM = full width at half height

 

HPLC Agilent 1100

 

The Agilent 1100 High Performance Liquid Chromatograph is used to analyze mostly polar non-volatile compounds. With the help of the device it is possible to determine different classes of biologically active substances: in particular, secondary plant metabolites (phenolic compounds, flavonoids, anthraquinones, saponins, etc.), phytohormones (auxins, gibberellins, abscisic acid and its derivatives, cytokinins, etc.), and other biologically active substances of plant origin (alkaloids, sesquiterpene lactones, etc.).

The main technical characteristics of the Instrument

Four-channel plunger pump (up to 400 bar) with flow rate of 0.001-10 ml/min (step 0.001 ml/min) with solvent compartment. Solvent supply adjustment step: 0.001 ml/min. The pH range of the eluent is 1…11.5. Plunger volume: 180 μl.

Vacuum degasser for 4 channels with a capacity of up to 10 ml / min.

Autosampler / Block of automatic sampling / (100 samples up to 2 ml) with the ability to add an internal standard, dilute the sample and perform derivatization. The possible volume of the sample is 0.1…100 μl. Input accuracy of 1% (relative standard error for the sample - 0.1 μl).

Column thermostat (temperature range from 10 °C below room temp. up to + 80 ° C (in steps of 1°). The thermostat holds 3 columns up to 30 cm long. Temperature maintenance accuracy ± 0.15ºC The thermostat is equipped with a device for recording and reading information stored on the identification modules of the columns.

Diode-matrix detector (1024 photodiodes) with a wavelength range of 190…950 nm (1 nm discreteness), with automatically variable programmable spectral width of the slit (from 1 to 16 nm.), including the in-process of chromatographic analysis, with a high pressure cuvette. The detector has an integrated holmium oxide filter to check the correct setting of wavelengths and is equipped with a system of warnings about the need for timely preventive replacement of the light source. Noise level during operation of the spectrophotometric detector is 0.6 × 10-5 units of optical density.

 

 

Terms of use of the Center's services

 

1. Researchers of institutions and organizations of the National Academy of Sciences of Ukraine who have the appropriate qualifications and experience on similar devices have the right to use the services of the Center.

2. Scientific organizations and institutions of the NAS of Ukraine that need to carry out the research at the Center, 1-2 months before the deadline submit to the head / responsible executive of the Center a standard application with a justification of scientific tasks and a description of the type and the class of compounds to be analyzed, indicate the number of samples.

3. The Customer and the Contractor enter into an agreement on carrying out works on the devices / equipment of the Center.

4. Provision (reimbursement) of consumables for a single cycle of work, sample preparation and depreciation costs are provided by customers.

5. For the convenience of collective use of devices/equipment and rational organization of working hours of the employees of the Center "Mass spectrometric complex with laser desorption / ionization MALDI-TOF Autoflex II LRF20", the following schedule of the Center is established: Tuesday - from 9.00 to 18.00 and Thursday from 9.00 to 17.00; lunch break - from 13.00 to 14.00. Customers can monitor the distribution of working time for scheduled research using the Center's instruments / equipment through an electronic calendar posted on the Center's website.

6. In the printed works presenting the results of research performed using the equipment of the Center “MALDI-TOF Autoflex II LRF20 Mass Spectrometric Complex with Laser Desorption / Ionization” Customers are obliged to refer to where and in what way the data were obtained, and generally to comply with copyright.

7. For customer institutions that are not subordinated to the National Academy of Sciences of Ukraine, the equipment of the Center “MALDI-TOF Autoflex II LRF20 Mass Spectrometric Complex with Laser Desorption / Ionization” may be provided on a commercial basis in accordance with the current legislation of Ukraine. Payment for consumables required for sample preparation and mass spectrometric analysis is determined with customers on a contractual basis.

8. Organizations and researchers who participate in the work and use the services of the Center are obliged to: comply with the Regulations of the Center "Mass spectrometric complex with laser desorption / ionization MALDI-TOF Autoflex II LRF20" of NAS of Ukraine; comply with the requirements of the head of the Center and his authorized persons on the organization of works and their safety.

 

 

Сalendar

 

 

Contacts

 

Head of the Center:

Volodymyr V. Turov, Deputy Director for Research, Corresponding Member of NAS of Ukraine, doctor of chemical sciences, professor

Tel.: +380 (44) 424 94 62, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.  

 

Responsible executives:

Taras Yu. Gromovyi, PhD in chemistry, senior researcher, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ;

Pavlo O. Kuzema, PhD in chemistry, senior researcher, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. , This email address is being protected from spambots. You need JavaScript enabled to view it. ;

Iryna V. Laguta, PhD in chemistry, senior researcher, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ;

Nadiia M. Moshkivska, leading engineer, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ;

Olga V. Severynovska, PhD in chemistry, senior researcher, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ;

Tetiana V. Fesenko, PhD in chemistry, researcher, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.  

 

Contact information:

Mailing address: 17 General Naumov Str., 03164 Kyiv, Ukraine

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Tel./fax: +380 (44) 424 94 56 / fax +38(044) 424 35 67.

 

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Conferences

 

Ukrainian conference with international participation 

"CHEMISTRY, PHYSICS AND TECHNOLOGY OF SURFACE"

devoted to the 35th anniversary of the Chuiko Institute of Surface Chemistry of NAS of Ukraine

and

Workshop

"NANOSTRUCTURES AND NANOMATERIALS IN MEDICINE: CHALLENGES, TASKS AND PERSPECTIVES" 

 

 

26−27 May, 2021, Kyiv

 

clipboard-list-outlineCONFERENCE PROGRAM

clipboard-list-outlinePOSTER SESSION ONLINE

clipboard-list-outlineBOOK of ABSTRACTS

clipboard-list-outlineONLINE BROADCAST (26 May)

clipboard-list-outlineONLINE BROADCAST (27 May)

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